Contributions

  • Köhler, Th. 1959- - Contributor

Publication

1995 - Springer, Berlin, Germany

Language

English

Word Count

41,250 words, Guess

Page Count

165 pages

Identifiers

  • ISBN-103540591923
  • ISBN-139783540591924
  • Goodreads4661896
  • Library of Congress Control Number95035772
  • Open LibraryOL18085335M

Classifications

  • DDC574.87/3283
  • LCCQP623.5.M47 O36 1995

Description

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e. g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; nRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

Subjects

Other Editions

  • Quantitation of mRNA polymerase chain reaction: nonradioactive pcr methodsSpringer1995-01-01

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