PCR/RT-PCR in situ light and electron microscopy
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Author
Contributions
- Raccurt, Mireille - Contributor
Publication
2003 - CRC Press, Boca Raton, Fla, Florida
Language
English
Word Count
104,000 words, Guess
Page Count
416 pages
Identifiers
- Internet Archivepcrrtpcrinsituli0000more
- ISBN-10084930041X
- ISBN-139780849300417
- Library of Congress Control Number2002073649
- OCLC Control Number50035168
and 2 more
- Better World Books9780849300417
- Open LibraryOL17058583M
Classifications
- LCCQH207 .M67 2003
- LCCQH207.M67 2002
Description
"PCR/RT-PCR in situ Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopic levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers, and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols."--BOOK JACKET.
Subjects
Topics
Series Statement
- Methods of visualization
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